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Fig. 1. Neural Grh expression is restricted to progenitors and its distribution is cell-cycle dependent. (A-C) Wild-type thoracic MARCM clones marked in green with nuclear ß-galactosidase (n-ßgal) or membrane-tethered GFP (mGFP) under tubulin-GAL4 control. Expression of Grh (red) is observed in neuroblasts (large dotted circles) and GMCs (arrowhead), but not in adult-specific neurons. (A) In interphase (I-phase) neuroblasts, Grh is excluded from the nuclelus and co-localizes with DNA (blue) in a crescent. (B,C) In mitotic (M-phase) neuroblasts, Grh is evenly distributed throughout the cell and does not specifically co-localise with DNA (blue in B) or phosphorylated histone H3 (H3p, blue in C), and is evenly distributed throughout the cell. In this and all subsequent figures, the neuroblast is indicated by a dotted circle.





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