FGF8 is required for cell survival at distinct stages of nephrogenesis and for regulation of gene expression in nascent nephrons
Development Grieshammer et al.
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Supplemental Figure 1
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Fig. S1. Analysis
of Fgfr2, Fgfr3 and Fgfr4 expression in E16.5 kidneys. (A-C) Cryosections of
E16.5 kidneys processed for in situ hybridization to localize the expression of
Fgfr2, Fgfr3 and Fgfr4,
as indicated. (A¢-C¢) Higher power views of the sections shown
in A-C. Fgfr2 transcripts are most
abundant in the collecting duct and are barely detectable in nascent nephrons.
The epithelia in which Fgfr3 and Fgfr4 are expressed were difficult to identify. CD,
collecting duct; NN, nascent nephrons; Po, podocyte progenitors; PZ, peripheral
zone; Tu, tubule progenitors.
Supplemental Figure 2
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Fig. S2. FGF8 is
not sufficient to induce nephrogenesis in isolated metanephric mesenchyme.
Metanephric mesenchyme was isolated from E11.5 wild-type embryos and cultured
(A) without additives, (B) with 200 ng/ml FGF8 (R&D Systems), or (C) in the
presence of dorsal spinal cord. After three days of culture, the samples were
processed for immunohistochemistry to detect phospho-Histone H3, which marks
cells in mitosis (blue), PAX2, which marks nephrogenic cells (green), and
E-Cadherin which marks nephron epithelia (red).
