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Fig. 4. Kidneys of Fgf8 hypomorphs are smaller than normal, but contain nephrons. (A) Comparison of normal, Fgf8^neo/neo (mild hypomorph) and Fgf8^neo/null (severe hypomorph) kidneys at E18.5. (B,C) In situ hybridization assay for Wnt4 RNA in vibratome sections of E15.5 kidneys. (D) Immunofluorescence assays at E15.5 for PECAM-positive (red) vasculature and WT1-positive (green) podocyte progenitors in vibratome sections. (D') A higher magnification view of a renal corpuscle in D.

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