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Fig. 6. Nephrons are truncated in severe Fgf8 hypomorphs and in cultures
of wild-type kidney explants treated with anti-FGF8 antibody. (A,C)
Immunofluorescence assays in vibratome sections of E18.5 kidneys from normal
and severe Fgf8 hypomorphs. Podocytes are marked by staining for WT1
(blue). Collecting ducts are marked by staining for both E-CAD (red) and
Calbindin (green), and thus appear yellow. The tubular portion of the nephron
is marked by staining for E-CAD only. Note that the cortex of the hypomorphic
kidney lacks the abundant, red tubular structures present in the normal
kidney. (B,D) Representative nephrons isolated from E18.5 kidneys better
reveal the lengths of the tubules. (E,F) Immunofluorescence assays for E-CAD
(red) to identify epithelia, and WT1 (green) to identify podocyte progenitors
of (E) control and (F) anti-FGF8 antibody-treated cultures of wild-type kidney
explants. (E',F') Higher magnification views. The black arrowheads
outlined in yellow indicate the proximal end of a tubule, where it is
connected to the renal corpuscle. The white arrowheads indicate the nearest
branch point of the collecting duct to which the tubule is connected. Note the
decrease in tubule length in the nephrons from the Fgf8 severe
hypomorph kidney and the anti-FGF8 antibody-treated cultures.
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