|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Expression of human PSEN1 protein in transgenic mice under the control of
the nestin intron 2 enhancer. Western blotting was performed on brain extracts
from E12.5 nestin-Psen1 transgenic (Tg/+) embryos from line 9 and line 14, as
well as non-transgenic controls (+/+). Blots were probed with NT.1 (upper
panel) an antibody that recognizes the 30 kDa N-terminal fragment (NTF) of the
transgenic human (Hu) PSEN1 but not the endogenous mouse (Mu) Psen1
(Wen et al., 2002b). The two
wild-type (+/+) embryos are immunonegative. The lower panel was probed with a
rabbit polyclonal antibody 222 that recognizes both the mouse and human NTFs
(Wen et al., 2002b). Antibody
222 was raised against amino acids 2-12 of the human NTF, a region that
contains one mismatch between the mouse and human proteins
(Wen et al., 2002b). Thus, the
apparent elevation in total Psen1 (Mu + Hu) in the transgenic (Tg/+) embryos
may in part reflect a higher affinity of this antibody for the human
protein.
|
