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Fig. 2. Ectoderm maintains the epithelial structure of somites. (A) Schematic of
the procedure: separation of the anterior PSM from overlying ectoderm by the
introduction of an impermeable membrane (blue), followed by 3-24 hours of
culture. The position of the membrane at the end of the experiment matches
perfectly the region where no staining is observed (see C-H). (B-D) Expression
of Fz7 after 3 (B), 12 (C) or 24 (D) hours of culture. The position
of the membrane is indicated in B. (E,F) Expression of Pax3 after 12
(E) or 24 (F) hours of culture. (G,H) Expression of paraxis after 12
(G) or 24 (H) hours of culture. (I,J) Transverse sections of C (I) and D (J)
at the positions indicated.