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Fig. 5. ß-Catenin regulates paraxis expression. (A) Schematic of the
experiment: embryos were halved with one half processed for Fz7
expression and the other half processed for paraxis expression, as
seen in D. (B) Schematic of the procedure: impermeable membranes were
positioned at each side of the embryo and the embryos were allowed to grow for
10 hours. Embryos were then cut in half and processed for Fz7 and
paraxis as before (E). (C) Somites (dorsal view) electroporated with
a DN ß-catenin construct lose paraxis expression in the
electroporated region (black arrowheads), whereas control somites express
paraxis in the entire dermomyotome (white arrowheads). (F,G) Dorsal
electroporation of DN Lef1 construct. Electroporated cells, which express GFP
(G), do not express paraxis (F).
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