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Fig. 1. Several bHLH factors pattern the early retinal neuroepithelium. (A) At
stage 14, HES1 transcripts accumulate in discrete domains in the eyecup (ec)
and neural tube (nt). There is no detectable accumulation of HES1 transcripts
in the central region (red bracket) of the presumptive retina. (B,C) The first
ATH5- and NGN2-expressing cells are detected in the central retina (nr) at
stage 15. (D) At stage 16, ASH1 transcripts are not detected in retina. (E) At
stage 17, a robust accumulation of HES1 transcripts is taking place throughout
the peripheral retina (J). In the central retina, a few cells located on the
vitreous side express HES1 at a high level (arrowheads in E,I). (F,K) Most
cells in the central retina express ATH5 and those expressing ATH5 strongly
are mostly located on the vitreous side. (H) At stage 18, the sparse cells
expressing Neuro M are scattered across the central retina (arrowhead in M).
There are no cells expressing ATH5 or Neuro M in the HES1 domain (F,H,L,N).
(O) Quantification of in situ hybridization. Adjacent retinal sections were
hybridized with the indicated bHLH riboprobes at stage 18. The ATH5, Neuro M
and NGN2 domains coincide in the central retina and they abut on the
peripheral HES1 domain. ASH1 is detected in an annular sector (G, brackets) at
the interface between the HES1 and ATH5 domains. l, lens. Scale bar: 140 µm
in A; 80 µm in B,C; 100 µm in D-H.
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