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Fig. 4. Transcriptional analysis of ATH5-expressing single cells. Stage 22-23
(E3.5) retinal cells were transfected with an ATH5-promoter/GFP-reporter
plasmid either singly or in combination with a vector expressing NGN2. They
were cultured for either 24 (E4.5) or 48 hours (E5.5). Stage 26 (E5) retinal
cells were transfected with the ATH5-promoter/GFP-reporter plasmid and
cultured for 24 hours (E6). Individual GFP-positive cells were collected and
processed for single-cell RT-PCR using the primers listed in Table S1 (see
supplementary material). (A) Representative transcriptional profiles obtained
with a set of 39 cells from the five groups generated by the experiment, as
identified by the colour code in B. RT-PCRs of total RNA isolated from E8
retina (NRE8) were used as positive controls for each set of primers. (C)
Ratios of HES1-, Neuro M-, Delta 1- and ASH1-positive cells to the total
number of cells tested for expression of these genes.
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