DEV01966 Supplementary Material

Files in this Data Supplement:

• Supplemental Table 1 - Adobe PDF

• Supplemental Table 2 - Adobe PDF

• Supplemental Figure 1 - Fig. S1. Differential expression and signal intensity plots for potential interacting genes in 5-week-old mouse mammary glands. Modified Log₂-Log₁₀ MA-plots in which arithmetic means and standard deviations were obtained after the fluorescence intensity ratios for TEBs or ducts versus distal stroma (M=Log₂(Cy5/Cy3)) and mean overall signal intensities (A=0.5[log₂(Cy5) + log₂(Cy3)]) were converted to linear values for each of six independent arrays are shown for the ERBB ligands on the TEB versus distal stroma (A) and duct versus distal stroma arrays (B) and for the ADAMs and TIMPs represented on the TEB versus distal stroma (C) and duct versus distal stroma arrays (D). The relative expression ratio of 1 (equivalent expression) and mean overall expression value of 2^A=256 (A=8, below which it was difficult to distinguish true signal from noise) are shown as broken lines. (A,B) AREG is expressed at high levels and relatively concentrated in TEBs and ducts. NRG4 is expressed at higher levels in the stroma than in TEBs or ducts. All other ERBB ligands are neither readily detectable nor highly expressed at high levels in any tissue compartment. (C,D) All ADAMs on the array are expressed; ADAM17 is equivalently expressed in stromal and epithelial compartments; TIMP1 is expressed at high levels in the TEB (but not duct) compartment; TIMP3 is more expressed ay higher levels in the stroma than in the TEBs. (E) The relative and absolute expression of TIMP1 is greater in TEBs and distal stroma than in ducts and distal stroma; the relative and absolute values for TIMP3 are lower in TEBs and distal stroma than in the ducts and distal stroma; ADAM17 is similarly expressed in ducts, TEBs and distal stroma.