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Fig. 6. EGFR phosphorylation in recombined mammary glands. Tissue extracts were
immunoprecipitated (IP) with anti-EGFR antibodies, immunoblotted (IB) for
phosphotyrosine (pTyr) or phosphorylated EGFR (pEGFR; Y1068), and re-probed
for EGFR. Western blots (WB) for keratin 14 were run on the original extracts
and re-probed for ß-actin. Renal transplants with the indicated
epithelial and stromal genotypes (wt, wild type; 17–/–,
Adam17–/–; A–/–,
Areg–/–; R–/–,
Egfr–/–) were harvested 2 weeks after
transplantation without exogenous estradiol. Control tissues were harvested
from 6-week-old wild-type male (M) and female (F) mice, and
Egfr–/– neonates (R–/–
p0) 15 minutes after intraperitoneal injection of EGF (2.5 mg/kg body weight)
or phosphate-buffered saline (PBS).
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