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Fig. 1. A genomics-based investigation into the nature and composition of a
neocortical protomap successfully identified genes with described spatial
differences in expression. (A) Strategy for a genomics-based identification of
protomap components. Rostral and caudal thirds of the neocortex were dissected
from mouse embryos at two ages (E11.5 and E13.5) in both inbred and outbred
strains. Comparison of gene expression between rostral and caudal tissue was
carried out within single litters of embryos to reduce variability in the
developmental stages of embryos. In addition to spatial gene expression, the
same samples were also used to analyse temporal changes in gene expression.
(B) Screen efficacy was demonstrated by the successful identification of
almost all of the genes that have been previously described as having
differential rostrocaudal expression, including COUP-TFI, Lhx2, Sfrp1
and Pax6. Plots of gene expression ratios (expressed in base 2)
between rostral and caudal neocortex are shown. The graphs show changes in
gene expression for single genes for multiple dye-swapped samples. The stage
and strain of origin of the samples is as indicated, with each datapoint
representing a measurement from a single microarray. Measurements are
presented as dye-swapped pairs, producing the alternating positive and
negative expression patterns. The two caudally expressed genes
COUP-TFI and Lhx2 show an opposite expression pattern to the
two rostral genes Sfrp1 and Pax6.
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