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Fig. 2. Diffusion of PcGFP in nuclei of preblastoderm embryos. A square-shaped
region (1.5x1.5 µm) in the center of a preblastoderm nucleus (A) of a
PcGFP embryo was photobleached and the fluorescence recovery was measured over
time. (B) Data points for 40 FRAP curves from similar nuclei as in (A) were
averaged and fitted to a hyperbolic function (Eqn 2 in the Materials and
methods). Scale bar: 5 µm.