DEV01943 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 - Fig. S1. Labeling of individual premigratory NC cells with iontophoresis at 13 hpf, and their descendants in the ANC. (A,B) NC dorsal to the eye form medial ethmoid. (C,D) NC posterior to the eye form trabecular cartilage.

• Supplemental Figure 2 - Fig. S2. A comparison of iontophoretic and PKH26 labeling of postmigratory NC cells at 22 hpf. (A,B,E,F,I,J) Iontophoresis; (C,D,G,H,K,L) PKH26. (A-D) NC at the anterior end of the head forms medial ethmoid. (E-H) NC beneath the eye forms lateral ethmoid and trabecular cartilage. (I-L) NC posterior to the eye forms trabeculae.

• Supplemental Figure 3 - Fig. S3. NC transplants from smu mutant donors migrate but do not form cartilage in wild-type hosts. (A) Schematic representation of transplantation. (B-E) Lateral (B) and dorsal (C-E) views of Alcian-Blue-stained mosaic larvae, whole mounted at 5 dpf. Transplanted mutant cells (brown) never form cartilage, unlike wild-type controls.

• Movie 1 - Movie 1. A 6-hour, confocal time-lapsed movie of a sox10:egfp transgenic showing cranial NC migration. Lateral view. Confocal stacks were collected every 10 minutes from 14-20 hpf, and projected at each time point. (A-C) Initially, labeled NC cells remain posterior to the forming optic vesicle, and then begin to migrate dorsal or medial to the optic vesicle. (D) Examples of migration paths of individual cells followed through stacks in this movie.