|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Expression of the sox10:egfp transgene in cranial NC cells of
living embryos. (A-C) Representative images from a confocal, time-lapsed movie
of sox10:egfp expression in the head between 14-20 hpf, lateral view
(see also Movie 1 in the supplementary material). (A-C) Brightfield (A) and
confocal images (B,C) at 14 hpf (A,B) and 20 hpf (C). (D) Diagram illustrating
migration paths of individual NC cells, traced by analyzing confocal stacks
within the movie. Circles indicate premigratory cell positions and stars
indicate their positions six hours later. Some cells (dark green triangle)
could not be tracked beyond 17.5 hpf, whereas others (light green square) were
only traceable after 16.5 hpf. (E-N) sox10:egfp+ cells at later
stages in wild-type (E-I) and Hh-deficient (J-N) embryos. (E) Lateral view at
24 hpf, showing the first pharyngeal arch (PA1) and stomodeum (arrowhead).
Precursors of the ANC lie beneath the eye and anterior to this stomodeal
pouch. (F,G) Ventral views with (G) and without (F) accompanying brightfield
images. (H,I) By 36 hpf, sox10:egfp+ cells aggregate around the mouth
(H), and, by 48 hpf, form cartilage of the ANC (I). (J) In Hh-deficient
embryos, sox10:egfp expression is similar to that seen in wild-type
controls, when viewed laterally. (K,L) However, in ventral view the head is
mediolaterally compressed, and NC cells anterior to PA1 fuse across the
ventral midline, between the eyes (arrow). (M) Fusion is more pronounced at 36
hpf, forming a single midline condensation (arrow). (N) A single rod of
sox10:egfp+ cartilage forms in the midline by 48 hpf. Scale bar: 50
µm.
|
