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Fig. 7. Exogenous Shh expands ANC cartilage in wild types and rescues syu
mutants. Ventral views, anterior to the left. (A-F) Flat-mounted,
Alcian-Blue-stained cartilages at 4.5 dpf. (G-I) Confocal images of
sox10:egfp expression in dissected embryos at 36 hpf. (A) Wild type.
(B) Wild type injected with shh mRNA. Trabeculae expand laterally and
often separate from the ethmoid (asterisk). (C) Wild type implanted with a
Shh-coated bead on the left side at 20 hpf. The ANC expands near the bead;
both trabeculae and ethmoid thicken, and the trabecula on the implanted side
fuses to the palatoquadrate (arrow). Inset shows the typical bead position
(arrowhead). (D) Control beads implanted at 22 hpf had no effect on ANC
formation in syu mutants. (E) Implantation of a Shh-coated bead
partially rescues the ANC in syu mutants. (F) Bead implantation at
30-36 hpf also partially rescues the ANC in some cases. (G) Control beads
implanted between 22-36 hpf had no effect on the distribution of
sox10:egfp+ cells in Hh-deficient embryos. Arrow indicates NC cells
at the midline. (H) Implantation of a Shh-coated bead at 22 hpf rescued
sox10:egfp expression at the ventral midline (arrowhead). Note that
the width of the bead-implanted embryos is identical to that of controls
(G,H), and smaller than that of wild type (I). Scale bars: 50 µm; in A for
A,B; in C for C-F; in I for G-I.
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