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Fig. 1. Gene trap integration into the C4st1 locus. (A) Schematic
representation of the integration of the PT-1 gene trap vector into intron 1
of the mouse C4st1 locus and the formation of a
C4st1-exonI-lacZ fusion transcript. (B) Embryos from intercrosses of
C4st1gt heterozygous animals were genotyped by Southern
analysis and the ratio of lacZ to en-2 alleles measured. (C-F)
Whole-mount in situ hybridization on E10.0 embryos using the indicated
exon-specific C4st1 probes. In branchial arches (white arrows) and
AER of limb buds (black arrowheads) in C4st1 mutant (gt/gt) embryos,
a C4st1 exon I-specific signal (E) is present, but an exon
II/III-specific signal (F) is absent.
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