DEV01978 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 - Fig. S1. The trunk vasculature develops normally in Gata4-depleted embryos. Shown are control (A,C) or morphant (B,D) embryos at 3 dpf, derived from transgenic fli1:GFP reporter fish, visualized under fluorescence for GFP expression in the vascular endothelium. C and D are higher order magnification views of A and B, respectively. Views are lateral, with dorsal to the top and anterior to the right.

• Supplemental Figure 2 - Fig. S2. Endocardium develops in Gata4,depleted embryos. Representative embryos derived from control (A) or morphant (B) tie2:GFP reporter fish at 2 dpf are shown. Bright GFP-positive regions of cushion development are evident in both the control and morphant embryos (indicated by C). Views are ventral with anterior to the right.