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Fig. 1. The Gata4 morpholino effectively blocks GFP translation in the gata4:GFP line, and generates a consistent phenotype. (A,C,E) Uninjected control fish; (B,D,F) embryos derived from eggs injected with MO. Living embryos are shown at 2 dpf in brightfield (A,B) or under fluorescence (C-F). (E,F) Control (E) and morphant (F) embryos at 3 dpf (lateral views, dorsal to the top with anterior to the right), demonstrating the complete block to GFP expression in both the notochord (N) and the heart (H).





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