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Fig. 6. Gut morphogenesis visualized in gata6:GFP transgenic embryos.
(Left) Representative control embryos that express GFP regulated by the
gata6 transgene at 2 dpf (A), 3 dpf (C) and 4 dpf (E,G). (Right)
Corresponding representative morphants from the same batch of transgenic
embryos as controls. Initial organ budding is evident at 2-3 dpf in the
morphant, in particular for the posterior (endocrine) pancreatic bud
(arrowhead). However, as indicated in D (arrowheads), the intestinal bulb,
liver and pancreas fail to grow. (A-D) Dorsal views with anterior to the
right; (E,F) lateral views with dorsal to the top and anterior to the right,
in order to visualize the pancreas; (G,H) lateral views with dorsal to the top
and anterior to the left, in order to visualize the intestinal bulb and liver.
E',F',G' and H' are higher order magnification views
of E,F,G and H, respectively. IB, intestinal bulb; L, liver; P, pancreas.
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