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Fig. 2. Distribution of RNF17 in male germ cells. Sections of adult testis were
immunostained with anti-RNF17 antibodies (anti-serum 1774, red). For purposes
of staging, testis sections were also stained with anti-SP-10 antibodies
(green) and counterstained with DAPI (blue). Only part of a seminiferous
tubule is shown in each panel. Anti-RNF17 staining alone is shown in
A,C,E,G,I,K. The merged image of anti-RNF17, anti-SP10 and DAPI is shown in
B,D,F,H,J,L. The stage of each seminiferous tubule is indicated in the center.
Representative RNF17 granules are indicated by arrows in pachytene or
diplotene spermatocytes, or by arrowheads in elongating spermatids.
Immunostaining of Rnf17-null testes with antiserum 1774 was negative
(data not shown). Control experiments without primary antibodies were also
negative (data not shown). Scale bar: 20 µm.
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