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Fig. 7. Targeted disruption of the Rnf17 gene. (A) Schematic diagram of
the wild-type allele, the targeting vector and the targeted allele. PGK-Neo:
neomycin-resistance gene driven by the PGK1 promoter. PCR primers
(P1, P2, P3 and P4) used for genotyping are indicated. (B) PCR analysis of
genomic DNA from Rnf17 mice. +/+, wild type; +/-, heterozygote; -/-,
homozygote. All four primers were included in each PCR assay. WT, wild-type
allele; MT, mutant allele. (C) Absence of RNF17 proteins in the
Rnf17-deficient testes. Western blot analysis was done with both
anti-sera 1774 (as shown) and GP8 (data not shown).
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