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Fig. 9. Analysis of gene expression in Rnf17-/- testes. ß-Actin was included as a control for RNA loading. (A) Northern blots of testis-specific genes in adult testes. +/+, wild type; +/-, heterozygote; -/-, homozygote. (B) Gene expression during juvenile testis development. Five testis-specific genes (Act, Tp1, Prm1, Prm2 and Ldh3) and four key regulators of spermiogenesis (Crem, Trf2, Miwi and Tpap) were examined. The age of mice (in days after birth) is indicated. +/+, wild type; -/-, Rnf17 deficient.





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