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Fig. 3. Physical association between Neur and DSL proteins. (A,B) Immunoprecipitations from transfected S2 cells expressing V5-tagged Dl (A) or myc-tagged Ser (B), along with nothing (lanes 1,4), EGFPneur (lanes 2,5) or neur{Delta}R-GFP (lanes 3,6). Lanes 1-3: cell extract (input). Lanes 4-6: anti-Neur immunoprecipitate. (C,D) Immunoprecipitations from larval disk/CNS complexes. (C) hs-Gal4; UAS-Dl-V5 along with another UAS transgene as follows: nothing (lanes 1,4), UAS-EGFPneur (lanes 2,5) or UAS-neur{Delta}R-GFP (lanes 3,6). (D) hs-Gal4; UAS-Ser-myc plus another UAS transgene, as in C. In lanes 4 of all panels (no Neur expressed) no DSL protein is detected, showing the specificity of the immunoprecipitation. In lanes 5 and 6, Neur and Neur{Delta}R, respectively, immunoprecipitate both Dl and Ser. Endogenous Neur protein is present in S2 cells, seen as a doublet in A and B (lane 4, asterisks). Transfected Neur produces higher molecular weight species owing to the GFP tags. Curiously the DlIC fragment was never detected in larval extracts. Molecular mass standards are shown in kDa to the right of each panel. FL, full-length; IC, Dl intracellular fragment; trunc, truncated Ser.





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