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Fig. 3. WNT signalling antagonises the formation of the pre-placodal region (PPR).
Primitive streak stage embryos were electroporated with GFP (A-E),
ß-catenin (F-H,J,K), WNT8C (I) or Crescent (L-Q), and analysed for PPR
and neural crest marker expression. Sections through control embryos (A-E) do
not show any change in the expression of Six1 (A), Six4 (B),
Eya2 (C), Pax7 (D) and Slug (E). Misexpression of
ß-catenin (F-H,J,K; brown) or WNT8C (I, brown) narrows (brackets in F-I)
Six1 (F), Six4 (G) and Eya2 (H,I) expression. By
contrast, Pax7 (J) and Slug (K) expression expands into the
placodal territory (arrowheads). Inhibition of WNT by Crescent (L-Q, brown)
expands Six1 (L,M), Six4 (N) and Eya2 (O)
expression into lateral (brackets in L,N,O) and trunk ectoderm (M, arrowhead),
while Pax7 (P, arrowhead) and Slug (Q, arrowhead) expression
is reduced.
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