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Fig. 6. Cooperation of FGF and BMP and WNT antagonists to induce the pre-placodal
region. Misexpression of SMAD6 and Crescent (A-D, brown in B,D) leads to an
expansion of Six4 (A,B; blue) and Eya2 (C,D; blue)
expression into the extra-embryonic epiblast. (E-G) FGF8 induces ectopic
Sox3 (E, arrowhead), Dlx5 (F, arrowhead) and Eya2
(H, arrowhead), but not Six4 (G, arrowhead). Misexpression of SMAD6,
Crescent and FGF8 (I-P) results in ectopic induction of Six4 (I,J;
blue) in the absence of the neural marker Sox2 (M,N) and of the heart
mesoderm marker Nkx2.5 (O,P). When the FGF8 bead is replaced with an
SU5402 bead after 5 hours (K,L), induction of Six4 expression is
still observed. Brown staining in J,L,N,P represents immunostaining with
anti-GFP antibody to visualise electroporated cells. (Q-T) Head mesoderm
(brown in R,T) was grafted into the extra-embryonic region together with
control (Q,R) or SU5402-coated beads (S,T). Six4 expression (blue) is
induced in controls (Q,R), but not when FGF signalling is inhibited (S,T).
Quail grafts are labelled using QCPN antibody (brown in R,T).
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