Supplemental Figure 1
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Fig. S1. Wild-type
and laterne seed. Wild-type seed (left) with lateral furrow
(arrowhead) separating cotyledons and hypocotyl. Cylindrical appearance of the laterne seed (right). Scale bar: 100 µm.
Supplemental Figure 2
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Fig. S2. Testing
for PINOID transcripts in laterne mutants. Single laterne,
pinoid and wild-type seedlings
were grown on 1/2 Murashige Skoog plates (at 18ºC, continuous light) and
sampled 1 week after germination. RNA was isolated from single seedlings (pinoid seedlings were recognised by their tricotyledoneous phenotype).
PINOID transcript was found in
all laterne seedlings tested
(three selected individuals shown). For control and comparison a b2/b3
chain TUBULIN transcript was amplified.
M1 and M2, molecular size standards; Ler 1 and Ler 2, wild-type
seedlings; pinoid, pid-15 +/pid-15 +
seedling; laterne 1-3, pid-15enp/pid-15enp
seedlings. The primers used for PINOID were 5´-CAGACGGTGAGATGAGTTTAGGAA-3´ and 5´-CCGGCGAAGACGAGGAAGA-3´. The
corresponding sequences are located in the first and second exon, respectively
(the band for mRNA/scDNA is 717 bp). The tubulin b2/b3-chain gene copy
was amplified with 5´-GAGATTCTTCACATCCAGGG-3´ and 5´-CATCTCGTCCATTCCCTCAC-3´
primers (the band for mRNA/scDNA is 1212 bp).
Supplemental Figure 3
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Fig. S3.STM,
ANT and CUC2 expression in pid-15
embryos. Cross-section series of tricotyledoneous pinoid embryos hybridised with STM (row A), ANT (row B) and CUC2 (row
C). Arrowheads point to broader STM
domain in A and correct CUC2
signal at the cotyledon borders in C. co, cotyledon. Scale bars: 20 µm.
Supplemental Figure 4
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Fig. S4.KNAT2::GUS and CUC3::GUS in wild-type, pinoid and laterne. (A,B) KNAT2::GUS staining
in wild-type (A) and laterne
seedling (B). (C-F) CUC3::GUS staining in wild-type (C), laterne (D,F) and pinoid embryos (E). (D) top view of laterne apical pole. (E) Arrowheads indicate stripe, which
is part of the CUC3 ring-shaped
expression between the central SAM and the cotyledon borders. Scale bars: 400
µm in A,B; 20 µm in C-F.
