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Fig. 8. Expression of STM, ANT and CUC2 in wild-type and
laterne (pid-15 enp) embryos.
(A,B,D,G,I,K,M,O,Q,R,T,U,W) Wild-type, (C,E,F,H,L,N,P,S,V,X) laterne,
(A-H) STM expression, (I-P) ANT expression and (Q-X)
CUC2 expression. (A-C,F,I-N,S,U,V,X) Arranged in series (top to
bottom). (A,B) Cross-section series (top view) of globular stage (A) and heart
stage embryo (B) with stripe-like domain of STM expression
(arrowheads). (C) Cross section heart stage (top view). (D,E) Lateral views of
wild-type (D) and laterne (E) heart stage embryos. (F) Tangential to
median sections of young laterne torpedo stage (black arrowheads
indicate patches of higher intensity). (G) Wild-type torpedo with STM
expression defining the SAM. (H) Comparable picture of laterne
torpedo, with patch-like pattern of STM signal. (I,K) Longitudinal
series showing the ring-like ANT expression pattern in globular stage
(I) and stained cotyledon primordia in heart stage (K). The black arrowheads
in I and K indicate the central region lacking ANT. (L) Longitudinal series of
laterne heart stage with ANT expression (arrowhead indicates
emerging apical cavity without signal; arrows indicate emerging expression in
vascular precursors). (M) Wild-type cross-section series with signal
concentration in vascular precursors. (N) Cross-section series shows extension
of ANT domain in apical pole of laterne, avoiding the centre
(arrowhead). (O) Longitudinal section of wild-type torpedo (slightly out of
centre; arrows as in L). Inset shows cotyledon cross-section. (P) Longitudinal
section of laterne torpedo (arrowhead: apical cavity lacks
ANT signal). Inset displays tangential cut to stress ring-shaped
ANT expression. (Q,R) Globular (Q) and early heart stage (R) each
with CUC2 expression. (S) laterne heart stage longitudinal
series with distributed patches of expression (arrowheads). (T) Wild-type at
late heart stage. (U) Wild-type cross-section series indicating the early
stripe-like pattern. (V) A laterne cross-section (arrowheads:
expression peaks); signal is also present in the centre. (W) Longitudinal
section in torpedo embryo with lateral (ring-shaped) signals, avoiding the
central SAM region (arrows). The inset is at a higher magnification. (X)
Series of torpedo stage laterne with distributed expression
(arrowhead). co, cotyledon; hy, hypocotyl; SAM, shoot apical meristem; vSAM,
variable SAM. Scale bars: 20 µm.
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