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Fig. 1. Genomic and molecular analysis of myt1. (A) An alignment of Myt1
sequences from Xenopus laevis, Homo sapiens and Drosophila
melanogaster. Black shading indicates identical amino acids and gray
shading highlights similar amino acids. The Myt1 protein sequence contains a
kinase domain (purple bar), a potential trans-membrane domain (green bar) and
a predicted C-terminal Cyclin B interaction motif (brown bar). (B) Physical
and genetic map of myt1 and flanking genes uncovered by
Df(3L)64D-F (white box). Black triangles indicate the position of the
nearest lethal P-element insertions that complement
Df(3L)64D-F. The black bar showing restriction enzyme sites (RI,
EcoRI; SI, SalI) represents a 24 kb DNA sequence
(coordinates 240,000 to 264,000) in AE003565, a genomic BAC clone. The narrow
black bar represents the DNA fragment that was subcloned to generate
P{myt1+}. Black arrow bars represent transcripts of
myt1+ and the nearest flanking genes. Roman numerals
indicate exons I to IV of the myt1 gene. (C) A single base deletion
mutation in myt11 (and myt12)
beginning at amino acid position 173 causes a frame-shift, producing an
altered amino acid sequence in Myt1 (red letters) that ends with a premature
stop codon at position 231.
