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Fig. 2. Cell proliferation defects observed in myt1 mutant testes. (A) Phospho-histone H3 (PH3, red) antibody staining of myt1/+ controls, shows mitotic cells at the apical tip (white arrow) and a cyst of meiotic cells (blue arrow) farther along the testis. No additional cells were labeled (yellow arrow). (B) PH3 staining of mitotic cells (white arrows) at the apical tip of the testes in myt1 mutants as well as additional 16-cell cysts of PH3-positive cells farther along the testes. Isolated mitotic cells (green arrows) were also found along the testis as well as a large cluster of labeled cells at the terminal end (yellow arrow). Scale bar: 20 µm.





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