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Fig. 7. Mitotic over-proliferation defects seen in myt1 mutant ovaries. (A,B) Ovarioles labeled by antibodies against PH3 (red) to mark mitotic cells, Hts (green) to mark spectrosomes and stained with Hoechst 33258, marking DNA (blue). In myt1 mutants (B), germaria often contained both dividing stem cells (green arrow) and dividing cystocytes (yellow arrow), not seen in controls (compare insets: a1, b1). The myt1 mutants also had more PH3-positive somatic follicle cells surrounding the egg chambers than did controls (compare insets: a2, b2). (C,D) Centrosomin staining (Cnn, red) for centrosomes, Hts (green) staining for fusomes and DNA (blue) staining showed significantly more metaphase stem cells (green arrow) and cystocytes (yellow arrows) in myt1 mutants (D) compared with controls (C). (E,F) Stacked confocal images of a Vasa-stained germarium, showing that myt1 mutant germaria (F) contain more cysts than controls (E). The myt1 mutant germline stem cells (F, green arrows) were also slightly smaller than controls (E). Scale bar: 40 µm for A,B; 8 µm for C-F.





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