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Fig. 8. Follicle cell defects observed in myt1 mutant ovarioles at
different stages of development. (A,D) Stage 2 egg chambers stained for Hts
(green) and PH3 (red). Stalk cells (arrow) are PH3-positive in 
90% of
myt1 mutants at this stage (D), but are PH3-negative in controls (A).
(B,E) Stage 7 egg chambers stained for PH3 (red). Follicle cells at each end
of the egg chamber (arrows) are PH3 positive in myt1 mutants (E) but
PH3 negative in controls (B). (C,F) Stage 7 egg chambers stained for Fas3
(green) and PH3 (red). Arrow indicates a PH3-positive polar cell (seen in 100%
of myt1 mutants, F), not seen in controls (C). (G,J) Stage 10A egg
chambers stained for Fas3 (green) and PH3 (red). Arrow in J indicates a
PH3-positive border cell (seen in 20% of myt1 mutants), not
present in controls (G). (H,K) Stage 10A egg chambers stained for Eya (green)
and PH3 (red). Arrows indicate PH3-positive, Eya-positive stretched cells in
16% of myt1 mutants (K) not seen in controls (H). (I,L) Stage
10B egg chambers stained for DNA (cyan) and PH3 (red). Arrows indicate
PH3-positive main body follicle cells in 6% of myt1 mutants (L)
but not in controls (I). Scale bar: 8 µm for A,D; 40 µm for B,C,E-G,J;
20 µm for H-L.
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