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Fig. 3. msh is required for the proper growth and patterning of the dorsal hinge. Third instar wing discs were stained (red channel) for the indicated proteins. In A,B,D,F, msh⁶⁸ M⁺ clones are marked by the absence of green. (A) Pattern of Wg accumulation. The expression seems only slightly decreased within the msh clone (white arrowheads). (B) Tsh accumulation is not appreciably modified by the msh clones. In A and B, the fold of the epithelium that separates the notum and hinge territories (blue arrowhead) is absent, but that which separates the hinge and the wing pouch (yellow arrowheads) is present. (C,D) Proneural clusters of Sc accumulation in a wild-type disc (C) and a disc with large msh⁶⁸ M⁺ territories (D). d, distal tegula; p, proximal tegula; r dorsal radius; a, anterior postalar cluster. Arrowheads indicate lateroanterior proneural clusters. (E) zfh-2 expression in the dorsal wild-type wing hinge (arrowheads). (F) zfh-2 expression is not modified in msh⁶⁸ M⁺ clones (arrowheads). (G) Overexpression of UAS-msh (green channel; ap-Gal4 driver, at 17°C) induces ectopic expression of zfh-2 in the wing pouch (arrowhead) and weakly in the notum territory (arrow).

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