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Fig. 5. Effects of arf6-2 and arf8-3 mutations on gene expression
in flowers. (A) Numbers of genes with expression that differs by at least
2.5-fold between different stages in wild-type flowers, grouped by stage of
highest expression, together with numbers of genes in each wild-type
expression class that were also developmentally regulated in arf6-2/arf6-2
arf8-3/ARF8 sesquimutant and arf6-2 arf8-3 double mutant
flowers. (B,C) Venn diagrams showing numbers of genes that were underexpressed
(B) or overexpressed (C) at different stages in arf6-2 arf8-3 double
mutant flowers, and in arf6-2/arf6-2 arf8-3/ARF8 sesquimutant flowers
(in parentheses). (D) Gene expression in response to auxin and jasmonic acid.
Wild-type or arf6-2 arf8-3 mutant mature flowering plants were
mock-treated or treated with IAA or JA for the times indicated, and blots of
RNA from inflorescence apices were hybridized with the indicated probes.
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