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Fig. 1. folded gastrulation and myosin localization. (A-C) Antibody
staining for fog protein (green) and cell outlines (Nrt,
red) in cross-section of ventral furrow (A), sagittal section of posterior
midgut (B) and apical surface of ventral cells (C). There is punctate
fog staining and localization towards the apical half of the cells.
(D-F) Antibody staining for fog protein in the region of the
posterior midgut in control shibirets embryos kept at the
permissive temperature (D), in shibirets embryos shifted
to the non-permissive temperature at gastrulation (E), and in embryos from
Rho-kinase germline clones (F). Punctate staining and apical
concentration of fog protein is reduced when endocytosis is blocked
using the non-permissive shibirets but is maintained in
Rho-kinase embryos despite the failure of the latter to form a
posterior midgut invagination. (G,I) Frames from time-lapse movies of
sqhGFP-expressing embryos 13 minutes after cellularization. In the
control (G), myosin is seen along the entire apical surface of the posterior
midgut (between arrowheads) and at the junctions of cells beyond this region.
In fog mutants (I), apical myosin is severely disrupted occurring in
only a few isolated cells underlying the pole cells (arrows). (H,J)
Cross-sections showing anti-myosin II antibody staining along the apical
surface of the ventral furrow (between arrowheads) in control OreR embryos (H)
and in fog mutants (J), in which it is present in some cells (+) but
not others (–).
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