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Fig. 2. Myosin localization in embryos misexpressing fog. (A-F) Staining for Fog
protein in control embryos expressing just the mat67;mat15 driver line (A-C)
and this line driving UASfog6 expression (D-F). At the
onset of cellularization (A,D), little fog expression is seen, but
early in cellularization, before nuclear elongation (B,E), there is high
fog expression throughout UASfog embryos. At the onset of
gastrulation, there is low fog expression in the ventral furrow of
control embryos (asterisk in C) and high expression throughout the
UASfog embryos (F). (G-L) Control (G-I) and
UASfog-expressing (J-L) embryos stained for myosin II. Myosin
localizes normally to the basal cellularization front and decreases normally
in ventral cells, which show the usual increase in cell depth (G,J). At the
onset of gastrulation, myosin continues to be localized basally and now also
localizes to the apical side of ventral cells in both control (H) and
UASfog (K) embryos. Apical myosin is more intense in UASfog
embryos and is no longer restricted to the ventral furrow, occurring in dorsal
and lateral cells (arrow) too. This continues through later stages of
gastrulation (comparing arrow in I and L). (M-O) Quantification of myosin
intensity in driver-line control (blue) and UASfog-expressing (pink)
embryos. Measurements were taken from the apical and basal sides of ventral
and lateral cells at three different stages: (M) end of cellularization (see
G,J), (N) onset of gastrulation (see H,K) and (O) later gastrulation (see
I,L). No difference is seen between control and UASfog embryos at the
end of cellularization (M) but during gastrulation (N,O) myosin increases on
the apical side of both ventral and lateral cells in UASfog embryos
with no corresponding decrease from the basal side. Error bars indicate
s.e.m.
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