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Fig. 4. Myosin localization in RhoGEF2 mutant embryos. (A-F) Localization
of myosin (white) to the cellularization front of Ore and RhoGEF2
mutant embryos (arrow in A,D). The cellularization front of RhoGEF2
mutant embryos is irregular at mid cellularization (E). (G-L) Localization of
myosin during gastrulation. For precise staging, embryos were viewed under oil
and individually fixed at the onset of gastrulation. Within this range of
ages, 41% (n=39) of Ore embryos accumulate myosin on the apical side
of ventral furrow cells (asterisk in H), but in RhoGEF2 mutant
embryos collected in the same way (n=30), myosin never accumulates
apically above background levels (asterisk in K), whereas basal loss of myosin
proceeds normally (arrow).
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