spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Myosin localization in Rho-kinase mutant embryos. Staining for Dorsal protein (red) marks ventral cells, nuclei are Hoechst stained (blue) and anti-myosin II antibody staining is in green. (A-F) Myosin localization in control embryos (A-C) and equivalently staged Rho-kinase (ROK) mutant embryos (below, D-F). ROK embryos have irregular incorporation of myosin to the cellularization front (+, myosin present; –, reduced myosin incorporation), irregular nuclear morphology and a failure to localize myosin to the apical side of ventral cells at the onset of gastrulation (compare asterisk in C,F). (G-K) Control embryos (G,H) and ROK mutant embryos (J,K) at later stages of development. Cells in later ROK mutant embryos are irregular (K). e, epidermal cell layer; f, folds; m, mesodermal cell layer. (I,L) nullo mutant embryos show both basal loss (arrow) and apical localization of myosin (asterisk) in ventral cells at gastrulation (L), despite earlier cellularization defects (I).





Right arrow Return to article