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Fig. 7. The role of adherens junctions in response to fog signaling. (A-G)
Staining of ventral (A-D,F) or lateral (E,G) cells showing the position of
adherens junctions (zona adherens, ZA) and basal junctions (BJ) stained with
anti-ARM antibody (red) and anti-myosin II antibody (M, green). (A-C) Movement
of junctions in Ore-R embryos: ZA are located subapically in all cells at the
end of cellularization (A) and this subapical position is maintained in
lateral cells, while ZA move to the very apical edge of ventral cells at the
onset (B) and during (C) gastrulation. (D,E) Control embryos from mat67;mat15
mothers show the same dynamics of ZA movement as seen in Ore-R, with junctions
located at the very apical edge of ventral cells (D) and subapically in
lateral cells (E) and at the onset of gastrulation. (F,G) Embryos expressing
UASfog6 from the mat67;mat15 driver line show an apical
shift of junctions in all cells both ventral (F) and lateral (G). Inset in E
and G: non-specific background staining has been amplified in the blue channel
to reveal the cell outline. (H-M) Localization of myosin II (green) to the
cellularization front of wild-type embryos, and embryos in which junction
formation has been disrupted (by expression of UASnullo, or by
germline clone reduction of arm protein). Neurotactin staining is
shown in red (except the arm panel in H where red staining is
non-specific cell surface stain). (H) Myosin localizes normally to the
cellularization front of wild-type, UASnullo and arm
germline clone embryos. (I,J) Myosin localizes normally to the apical surface
of ventral cells (arrow) at gastrulation in wild-type (I) and
UASnullo (J) embryos. (K-M) Images of the apical surface of ventral
cells. Cells are outlined by Nrt staining (red). Myosin (green) is
localized throughout the apical surface of wild-type cells (K) but is
constricted to a tight ball (arrow) within the cells of UASnullo (L)
and arm germline clones (M), leaving large black (non-stained) areas
without myosin that are not seen in wild type.
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