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Fig. 5. (A) High-level chimerism could be detected in all major adult haematopoietic organs following the transplantation of VE-cadherin-expressing HSC candidates from the E11.5 AGM region, E12.5 peripheral blood and E13.5 foetal liver. There is significant contribution to short-lived granulocytes/monocytes (MAC1+GR1+) and pro/pre-B-cells (B220+CD43+) within the bone marrow, and immature CD4+CD8+ thymic T-cells 12 weeks after transplantation. (B) Successful haematopoietic reconstitution of secondary recipients demonstrates the long-term self-renewal potential of VE-cadherin+ stem cells. The percentage of cells in each gate/quadrant is indicated.





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