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Fig. 1. (A) Splicing pattern of nematode fibulin-1C and fibulin-1D. Exons are
numbered as in Barth et al. (Barth et al.,
1998) with the exception of exons 5 and X, which are labeled 5C
and 5D, respectively. Horizontal line indicates region deleted in
fbl-1(hd43). Arrows indicate region where GFP or YFP tags were
introduced in the fibulin coding sequence. Arrowheads indicate region where
mutations were introduced to inactivate specific splice variants (see
materials and methods for details). (B) RT-PCR experiment demonstrating that
exon 5C is detected in the fibulin-1C variant only, and is replaced by exon 5D
in the fibulin-1D variant. Forward and reverse exon specific primers are
listed respectively. Lane 1, exons 5C and 14; lane 2, exons 5C and 16; lane 3,
exons 5D and 14; lane 4, exons 5D and 16.
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