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Fig. 3. The patch compartment, as well as its dopaminergic innervation from the midbrain, is significantly impaired in the absence of Notch1. (A) Patch neurons in the developing striatum begin to aggregate and display cell-specific markers at E18.5. Immunostained adjacent coronal forebrain sections show that glutamate receptor1 (Glur1) and Darpp32 are expressed by newly differentiating subcallosal (SCS) and patch neurons (arrows) in wild-type embryos but are abnormally expressed in Foxg1Cre; N1 cKOs. The SCS is thicker in the Foxg1Cre; N1 cKOs than wild-type embryos relative to the overall size of the striatum. Scale bar: 200 µm. (B) Incoming dopaminergic fibers from the substania nigra (SN) express tyrosine hydroxylase (Th) and become selectively localized to SCS and patch neurons in the wild-type striatum (arrows). Foxg1Cre; N1 cKOs, which display an expanded SCS, show greater Th innervation than do wild-type mice at E18.5 (upper panels). In addition, fibers from the SN ectopically project to the cerebral cortex in the absence of Notch1. Excessive Th-positive fibers are present throughout the prefrontal cortex in Foxg1Cre; N1 cKOs whereas Th-positive fibers are rarely observed in the wild-type cortex at E18.5 (lower panels). Scale bar: 100 µm.

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