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Fig. 6. The ciliary funnel expresses orthologs of placode-marking genes. Left lateral (A) and dorsal (C) views of early hatchlings shows that the roof of the pharynx expresses six3/6a. six3/6a is also expressed in the anterior brain, rostral towards the sensory vesicle. Left lateral (B) and dorsal (D) views of the same stage as in A,C show that six3/6b is expressed in a more limited domain in the right side of the pharynx roof in just three cells. When the ciliary funnel can be easily seen as a projection to the right of the brain in late hatchlings, six3/6a (E) and six3/6b (F) are both expressed in the funnel walls. In dorsal (G) and lateral (H) views of late hatchlings, pitx expression can be seen in both the dorsal and ventral walls of the pharynx (ph), as well as in the ventral-most part of the funnel wall where it meets the pharynx. br, brain expression; cf, ciliary funnel; ep, epidermal expression; lb, bristle-bearing lip cell; mo, mouth; ph, pharynx. Scale bars: 10 µm.





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