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Fig. 3. FGF signalling is insufficient for stem zone specification. FGF8 soaked bead in extra-embryonic epiblast at HH3 (A). bra is induced by FGF8 within 4 hours (B) (high magnification in C), but stem zone gene Sax1 (D) is only detected after 10 hours. 4b explants do not express Sax1 following exposure to 200 ng/ml FGF4 (E,F) or to 250 ng/ml FGF8 (G,H), but can express bra following exposure to 200 ng/ml FGF4 (I,J) or to 250 ng/ml FGF8 (K,L). Scale bar: 100 µm.





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