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Fig. 5. Prolonged expression of Cut maintains follicle cells at an immature stage.
Cut-misexpressing cells are marked with co-expression of GFP (green in
A,B,D,F) or Cut staining (green in E,G). (A,A') FasIII (red in A and
white in A') continued to be expressed in follicle cells overexpressing
Cut (outlined) in stage 9 egg chamber, whereas neighboring wild-type follicle
cells no longer expressed FasIII, except in the polar cells (arrowhead) and
the stalk cells (not shown). (B,B') Big brain (Bib, red in B and white
in B'), a stalk-cell marker, was expressed in the stalk cells (arrow)
but not in the main-body follicle cells even if Cut was overexpressed. (C) The
Eya expression pattern during early oogenesis. Eya protein (shown in red) was
expressed in the germarium and in all follicle cells except the polar cells up
until stage 8, but the expression was lower after stage 6. PH3 (shown in
green) was used to indicate the M phase of the mitotic cycle. (D,F) Eya (red
in D,F and white in D',F') was continuously expressed in follicle
cells overexpressing Cut after stage 7 and until stage 10. Eya was not
expressed in stage 12 egg chambers (F,F' with increased background).
(E,G) Polar-cell fate marker A101 (stained with anti-ß-gal, red in E,G
and white in E',G') was only expressed in polar follicle cells
(arrowhead in E and E') in the middle stage, whereas it was expressed in
main-body follicle cells overexpressing Cut after stage 10.
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