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Fig. 1. Tou displays homology with members of the WAL family of chromatin
remodelling factors. (A) Physical map of the tou locus. Structure of
the tou gene and locations of the transposon insertions in EP662,
tou1 and EY08961 are shown. tou2
and touE44.1 were generated by imprecise excision of
tou1 and EY08961, respectively. The transposon in
EP622 contains UAS sequences that allow overexpression of
tou with the UAS/Gal4 system. Noncoding and coding regions of the
tou transcript are depicted as open and filled boxes, respectively.
(B) Tou shares functional domains with proteins expressed in
Drosophila (Dm Acf1) and in human (Hs TIP5; Hs WSTF) and involved in
chromatin remodelling. Tou carries a MBD domain (methyl-CpG binding domain), a
DDT domain (after DNA binding homeobox and different transcription factors), a
WAKZ motif (WSTF/Acf1/KIAA0314/ZK783.4), two PHD fingers (plant homeodomain)
and a C-terminal bromodomain (BROMO). TouA, isolated during the yeast
two-hybrid screening, contains the MBD and DDT domains. Dm Acf1 and Hs WSTF
lack the MBD but display a N-terminal WAC motif (WSTF; Acf1; cbp146).
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