QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 1. Partial rescue of the skeletal growth in the DKO embryo at E18.5. (A1-A5) Forelimbs of embryos of various genotypes: wild type (A1), Gli3^-/- (A2), DKO (A3), Ihh^-/-; Gli3^+/- (I^-/-; G^+/-, A4) and Ihh^-/- (A5). (A1) s, scapula; h, humerus; r, radius; u, ulna; mc, metacarpal; p, phalanges; ^*, deltoid tuberosity. (A2) Numbers 1-7 indicates polydactyly. (A3) Red arrowhead denotes bifurcation of the proximal phalange (P1). Inset shows higher magnification of boxed region. Green arrowhead indicates ectopic bone. (B1-B5) Hindlimbs from embryos of various genotypes: wild type (B1), Gli3^-/- (B2), DKO (B3), Ihh^-/-; Gli3^+/- (I^-/-; G^+/-, B4) and Ihh^-/- (A5). (B1) fe, femur; t, tibia; fi, fibula; mt, metatarsal; p, phalanges. (B2) Numbers 1-6 indicates polydactyly. (B2,B3) Big red arrowheads indicate truncation of the tibia (t^*). Small red arrowhead indicates bifurcation of the proximal phalange (P1). Inset shows higher magnification of boxed region. Green arrowhead indicates ectopic bone. (C1-C4) Rib cages from embryos of various genotypes: wild type (C1), Ihh^-/- (C2), DKO (C3) and Gli3^-/- (C4). Double-headed arrows (C1-C4) indicate the width of the rib cage. Asterisks (C3,C4) indicate the split sternum. (D1-D3) Skulls of embryos of various genotypes: wild type (D1), Ihh^-/- (D2) and DKO (D3). Asterisks (D1-D3) denote the chondrocranium. (E) Detection of Gli3 in limb cartilage proteins. Gli3^A, the full-length (190 kDa) activator form of Gli3; Gli3^R, the truncated (83 kDa) repressor form of Gli3. ${alpha}$ -cat., ${alpha}$ -catenin (102 kDa) used as normalizer. Inset shows longer exposure of the Gli3^A band. Asterisk indicates a non-specific protein (80 kDa) reacting with the antibody. (F1-H2) In situ hybridization using ³⁵S-labeled riboprobes against Gli1 (F1,F2), Gli2 (G1,G2) and Gli3 (H1,H2) on longitudinal sections through the ulna (F1,F2,H1,H2) or the humerus (G1,G2) from E14.5 wild type (F1-H1) or Ihh^-/- (F2-H2) embryos. Shown is the distal region of each element. Green arrows indicate expression in the perichondrium. Green asterisks indicate the periarticular region. P, proliferative chondrocytes; H, hypertrophic chondrocytes. r, h, u (F2,H2) indicate radius, humerus and ulna, respectively.

Return to article