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Fig. 5. Tbx1 is required during pharyngeal segmentation. (A-C) Coronal sections of X-gal stained E10.5 Tbx1^mcm/+;R26R embryos exposed to TM at E7.5 (A), E8.5 (B) and E9.5 (C); cranial is upwards. Arrowheads indicate the 3rd pharyngeal pouches, and arrows the 4th pharyngeal pouches. There is a progressive increase in number of labeled endodermal cells in the caudal segments. (D-F) External aspect of E10.5 TgCAGG-CreER^TM;Tbx1^flox/- embryos exposed to TM at E7.5 (D), E8.5 (E) and E9.5 (F). External abnormalities were only detected in embryos exposed to TM at E7.5 (D), which showed severe hypoplasia of the 2nd pharyngeal arch, II. (D'-F') Intracardiac ink injection of the embryos in D-F to visualize the pharyngeal arch arteries (PAAs). Numbers indicate the PAAs. The left 6th PAA in E' is absent, while the right one appears normal. (G-I) Coronal sections of E10.5 TgCAGG-CreER^TM;Tbx1^flox/- embryos exposed to TM at E7.5 (G), E8.5 (H) and E9.5 (I); cranial is upwards. Arrowheads, 3rd pharyngeal pouches; arrows, 4th pharyngeal pouches, black stain is residual ink injected into these embryos before paraffin embedding. (J-L) Left view of E10 TgCAGG-CreER^TM;Tbx1^flox/- embryos exposed to TM at E7.5 (J), E8.5 (K) and a Tbx1^flox/- control (L) hybridized with a Pax1 probe to identify the 1st (I), 2nd (II) and 3rd (III) pharyngeal pouches. Scale bars: 0.5 mm in F; 0.2 mm in F' and L; 0.1 mm in C and I.

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