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Fig. 2. Targeted disruption of the Shox2 gene in mice. (A) The mouse
Shox2 genomic structure spans 8.3 kb, and contains 6 exons, as
indicated by numbers. The targeting vector contains genomic fragments flanking
the PGK-neo cassette which replaces exon 3 of Shox2 when
correct homologous recombination occurs in ES cells. (B) Southern blot
analysis of genomic DNA (digested with BamHI and probed with
3'-probe) derived from E11.5 embryos with different genotypes. (C) PCR
analysis of DNA from DNA samples from yolk sac of embryos using W and M pairs
of primers for wild-type and mutant alleles, respectively (see A). The W set
of primers amplify the wild-type DNA fragment at the size of 520 bp, while the
M set of primers amplify the mutant DNA fragment of 300 bp. (D) RT-PCR assays
showing the absence of partial Shox2 transcripts in
Shox2-deficient embryos.
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