DEV01553 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Timelapse imaging using sagittal explant culture technique reveals dynamic migratory behavior of neural crest cells as they form the DRG and SG. Thirty-six hour timelapse, 10 minute intervals, 103 objective.

• Movie 2 -

  Movie 2. Neural crest cells disperse along the dorsal aorta before forming discrete SG. Five-hour timelapse, 3 minute intervals, 103 objective, zoom=1.5.

• Movie 3 -

  Movie 3. Filopodial contacts among neural crest cells assist in their dispersion along the dorsal aorta and in discrete gangion formation. Thirty-six hour timelapse, 10 minute intervals, 103 objective.

• Movie 4 -

  Movie 4.  High resolution time-lapse revealing dynamic cell behavior of cells positioned between two ganglia as SG segregate. 3.5-hour timelapse, 1 minute intervals, 403 objective.